A long but interesting read: The Sugar Conspiracy.

If you had ever seen the not-so-descriptive error message
A required CD/DVD drive device driver is missing,
then you have been trying to install Windows 7 (possibly using a bootable flash drive) on a recent laptop or desktop.

There are two major obstacles for a somewhat-dated Windows 7 when it sees modern hardware:

• USB 3.0
• SSDs and newer disk drives in general

Fortunately, both problems are easy to fix.
Just follow the steps below; skip steps 1 and 2 if you already have a bootable Win7 flash drive.
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A while ago I was looking for GTD/TSW-compatible android app.
I ended up using Trello, Keep, and Calendar.

But I always keep looking for new/improved tools, as right now I feel the best one does not exist…
(If the best one can exist at all – requirements and conditions change all the time, so there is no fixed perfect immovable target.)

I have been contemplating trying out the TSW methodology, but neither Keep nor Trello are quite there yet.
I ended up using Evernote; after recent management changes and actually trying to become profitable it may as well last long enough.

Everything was fine and calm until I have found workflowy yesterday.
In essence, it is very similar to the text-file-based system that I have been using for at least half a year.

Briefly, it is a web-based text editor on steroids, with possibly infinite nesting lists and seemingly full keyboard shortcuts control – no mouse needed.
I recommend that you try the demo – it seems to be fully functional, and there is no need to sign up.

This discovery made me read through pages and pages of this class of software tools.
Here is a very brief summary of my findings: Read the rest of this entry »

I used to work with sequencing providers who were giving me fairly clean data.
It was already barcode-separated, and had no over-represented adapter sequences.
The only thing I had to do was to (optionally) quality-trim the reads, and check for biological contamination.

Recently, however, I have come across some real-world data, which not only had contamination in it, but also quite a noticeable percentage of adapters.
I did a quick test of multiple tools to see if they fit my requirements:

• should be easy/logical to use: no arcane/convoluted command lines or config files
• should detect adapters automatically, either using its own database or a provided plain FASTA file
• should be reasonably fast
• must leave no adapter traces behind: I prefer aggressive trimming

I have tried the following tools:

• fastq-mcf from the ea-tools package
• skewer
• TrimmomaticPE
• cutadapt: haven’t used it directly, but it is used by some of the compared tools
• bbduk from BBMAP
• autoadapt
• TrimGalore!

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