wget ––continue ––mirror ––page-requisites ––adjust-extension ––convert-links ––backup-converted ––limit-rate=500k ––wait=2 URL

(short form: wget -cmpEkK ––limit-rate=500k -w 2 URL)
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Here’s a simple and clear guide for gmail, which also definitely works with other relay hosts. I’ve used it to configure my ISP’s mail relay (they block outgoing port 25) on a Debian Squeeze laptop.

Imagine you need to get a few lines from a group of files with missing identifier mappings. I have a bunch of files with content similar to this one:

ENSRNOG00000018677 1368832_at 25233
ENSRNOG00000002079 1369102_at 25272
ENSRNOG00000043451 25353
ENSRNOG00000001527 1388013_at 25408
ENSRNOG00000007390 1389538_at 25493

In the example above I need '25353', which does not have corresponding affy_probeset_id in the 2nd column.

It is clear how to do that:

This outputs a column of required IDs (EntrezGene in this example):

116720
679845
309295
364867
298220
298221
25353

However, I need these IDs as a comma-separated list, not as newline-separated list.

There are several ways to achieve the desired result (only the last pipe commands differ):

These solutions differ in efficiency and (slightly) in output. sed will read all the input into its buffer to replace newlines with other separators, so it might not be best for large files. tr might be the most efficient, but I haven't tested that. paste will re-use delimiters, so you cannot really get comma-space ", " separation with it.

Sources: linuxquestions 1 (explains used sed commands), linuxquestions 2, nixcraft.

1. Install the annotationTools R package:
   source("http://bioconductor.org/biocLite.R")
   biocLite("annotationTools")
2. Download full HomoloGene data file from ftp://ftp.ncbi.nlm.nih.gov/pub/HomoloGene/current
3. library(annotationTools)
4. homologene = read.delim("homologene.data", header=FALSE)
5. mygenes = read.table("file with one entrez ID of the source organism per line.txt")
6. getHOMOLOG(unlist(mygenes), taxonomy_ID_of_target_organism, homologene) [alternatively, wrap the call to getHOMOLOG into unlist to get a vector]

It might be easier to achieve the same results with a Perl script calling NCBI's e-utils.

Source: Kelly's think tank.

Find out more about the depicted office. That would be a nice setup for flight and/or space simulators, I guess.

TinEye Firefox extension helped finding more nice workplaces.

And Stefan in his office description provided some more links to multi-display workplaces - Mitch Haile's and Kevin Connollie's among others.

Just a quick note: upgrading Drupal using a patch file is a really efficient and fast method, especially because diff/patch files are available for different Drupal version combinations.